Evaluation of Maturity and Ovarian Development of the Hybrids of African Catfish, Clarias gariepinus and Indian Catfish, Clarias batrachus in Comparison to Their Control Siblings
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Abstract
Histological study to evaluate maturity of four crosses of African catfish, Clarias gariepinus and Indian magur, Clarias batrachus (Cg ♀x Cg ♂, Cb♀x Cb♂, Cg ♀x Cb♂ and Cb♀ x Cg ♂) was carried out by dissecting the ovaries and examining the stage of oocyte development on fishes reared for one year. The sacrificed fishes were fixed in 10% neutral buffer formalin solution (NBF). The ovaries sampled for histology were rinsed to eliminate excess formalin solution, dehydrated in an ethanol series of progressive concentrations, cleared in a solvent and embedded in paraffin. Then 5-μm thick transversal sections were made with a rotary microtome followed by staining with haematoxylin and eosin. Sections of the ovaries were histologically analysed to investigate differences among the breeds. The results indicated that the ovaries of both types of hybrid (Cg ♀x Cb♂ and Cb♀ x Cg ♂)crosses were comparable, more or less mature uniformly at well progressed stages (stage 5 and 6) in contrast to the pure Indian magur progenies (Cb x Cb). Although little variation is observed, Cb x Cb appeared more or less in a similar oocyte developmental stages with the pure breed of C. gariepinus (Cg x Cg) used as a control in the experimental design. Of all the ovaries examined, the pure C. batrachus progenies were observed at their lower stage of oocyte development indicating that this group crosses mature too late compared to all the other siblings of the same age groups of individuals.This is usually related to their small size.
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References
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